Follicular Fluid Oxidative Stress Biomarkers and Intracytoplasmic Sperm Injection (ICSI) Outcome

Abstract

The aim of this study was to evaluate the association between the follicular fluid (FF) malondialdehyde (MDA) levels as a free radical marker and reduced glutathione (GSH) as antioxidant and pregnancy after intracytoplasmic sperm injection (ICSI).A total seventy-five infertile women aged between 22-45 years(31.43 ± 5.38 years), referred to the fertility clinic in Al-Sadder teaching hospital, , and undergone intracytoplasmic sperm injection throughout period from March 2013 to January 2014,were included in this study. follicular fluid (FF) malondialdehyde was measured by spectrophotometer based on the calorimetric reaction with thiobarbituric acid (TBA) and follicular fluid glutathione was measured by spectrophotometer depends on the action of sulfhydryl groups.Out of the 75 included patients, 15 (20%) achieved pregnancy after ICSI. Pregnant women were associated with significantly higher MDA level (P < 0.05) and lower GSH (P < 0.05) .There was a significant positive correlation between MDA and the number of pronucleus ( r= 0.326 ). The best cutoff value of FF.MDA and FF GSH associated with pregnancy were 3.49 µM and 26.90 µM respectively. Higher FF MDA and lower FF GSH levels are associated with pregnancy after ICSI. Oxidative stress parameters may be markers of metabolic activity within the follicle.