Increasing the Production of Spilanthol in Spilanthes acmella (L.)Murr. by Using Tissue Culture Techniques


This study was aimed to increase the production of Spilanthol from S. acmella by using tissue cultures. Seeds were sterilized and cultured on MS medium supplemented with different concentrations 2.0,4.0,6.0 mg/l of Naphthalene acetic acid (NAA),1.0 , 2.0 , 3.0 mg/l Benzyl adenine (BA)and 1.0, 2.0, 3.0 mg/l Benzyl amino purine (BAP). These growth regulaters were used for callus induction from leaf segments, plus 0.1 gm casein hydrolysate in combination with the same growth regulaters for callus induction and Spilanthol production. Quality and quantity of Spilanthol were investingated using high performance liquid chromatography (HPLC), results showed that using 1.5% sodium hypochlorate for 15 min was very effective for disinfecting and survival . The combination of 3.0 mg/ l BAP, 6.0 mg/l NAA with 0.1 gm casein hydrolysed was most effective for callus induction from leaf segments ,The highest mean fresh weight and the highest amount of Spilanthol reached to 80.46 mg/g in combination of 2.0 mg/lBAP, 2.0 mg/l NAA with 0.1 g casein hydrolysed. This increased the Spilanthol compound to its highest amount when compaired with the original plant.