Salivary microRNAs (hsa-miR-200a, hsa-miR-125a and hsa- miR-93) in relation to age, gender and histopathological parameters


Background: MicroRNAs (miRNAs) are small noncoding RNAs that post‐transcriptionally regulate gene expression bytargeting specific mRNAs. The main objective of this study was measure the level of salivary (hsa-miR-200a, hsa-miR-125a and hsa- miR-93) in both oral squamous cell carcinoma and healthy controls to asses the association of themwith age, gender and tumor gradematerials and methods The level of three salivary microRNAs namely hsa-miR-200a, hsa-miR-125a and hsa- miR-93were measured in saliva of patients with oral squamous cell carcinoma and healthy controls by using reverestranscription, preamplification and quantitative PCR also the general information from each patient including theage, sex and tumor grade were recorded.Results: Salivary miR-200a was down regulated while miR-93 was up regulated in saliva of females with OSCCcompared to females of healthy control, also there was a weak and statistically insignificant positive linearcorrelation between tumor grade and hsa-miR-200a CT values (r=0.223).However each tumor grade group had amean normalized CT value which was higher than that of controls with statistically significant differences, P<0.05. Theresults suggest that circulating miRNAs may be a biological marker of aging and tumor grade. More studies shouldbe done to validate these results.Conclusions: Both miR-200a and miR-93 could be used as biomarkers for poorly differentiated and aggressive cancer