Immunohistochemical expression of MMP9, as a marker of local invasion in Hodgkin’s and Non-Hodgkin’s lymphoma of the head and neck region


Background: Malignant lymphoma is the generic term given to tumors of the lymphoid system and specifically oflymphocytes and their precursor cells, While all lymphomas are malignant neoplasms, there is a wide spectrum ofclinical behavior, with some following an indolent clinical course and others behaving in an aggressive manner (willcausing death in a short time frame if left untreated). The metastatic process involves intravasation andextravasation of tumor cells, followed by reimplantation of tumor cells, formation of a new tumor stoma, Degradationof the extracellular matrix and components of the basement membrane by proteases facilitates the detachment oftumor cells, their crossing of tissue boundaries, and invasion into adjacent tissue compartments. The importance oftumor-associated proteases in invasion and metastasis has been demonstrated for a variety of solid malignanttumors. MMP family has been implicated in tumor cell invasion and metastasis.Material and methods: This study included 67 formalin-fixed, paraffin-embedded histopathologically diagnosedlymphoma blocks (head and neck lesions), 24 Hodgkin’s Lymphoma and 43 Non Hodgkin’s Lymphoma.Immunohistochemical (IHC) evaluation of MMP-9 monoclonal antibodies), in relation to the clinicopathologicalparameters was assessed.Results: The mean of expression of MMP-9 in NHL was higher (65.4±18) than that in HL (56.7±21.4) though it did notreach the level of statistical difference P > (0.05), while the mean of expression of MMP-9 in relation to tumor gradeswas different as it had a value of (72±14.1) in low grade tumors, (68.3±15) in the intermediate grade tumor and(61.4±20.7) in high grade tumors; consequently, this difference did not reached the level of statistical significanceP(ANOVA) >0.05 . MMP 9 had no statistical significant correlation in regard to its mean in HL subtypes.Conclusions: This study had shown that there was no significant correlation between age and mean of expression ofMMP-9 in HL and NHL. There was no statistical significant difference in the mean of expression of MMP-9 between HLand NHL subtypes