أستخلاص وتنقية الفلافونويدات من البروبوليس وتقييم فعاليتها ضد بعض انواع الاحياء المجهرية المرافقة لتسوس الاسنان

Abstract

Extraction conditions for two types of flavonoids [ Flavone/ Flavonol, (FF)and FlavanoneDihydroflavonols, (FD)] from propolis were optimized .The results revealed that 70% ethanol as extraction solvent with 96 hours extraction time gave the highest yield of flavonoids . The menthioned flavonoids were purified ,the purification steps included extraction with 95% ethanol and adsorption chromatography using sephadex LH-20 . The purification procedure resulted in (19.31%) yield for the flavonoids. The inhibitory activity of ethanolic propolis extract was evaluated against some microorganisms associated with dental caries .The tested microorganisms included eight species of the genus Streptococcus isolated from human mouth as well as the yeast Candida albicans .The results showed that the propolis extract gave strong inhibitory activity against the microorganisms used in this study . The synergsim effect of propolis and the antibiotic Chlorohexidine (CHX) was studied against two types of Streptococcus .Results showed that inhibition zone against S.mutans was increased from 18 mm to (21 ,21.5 and 24) mm with the addition of (0.01,0.1 and 0.2)% of the CHX , respectively. The same inhibitory effect was noticed against S. salivarius when the synergism action of propolis with CHX was studied .