Molecular Localization of Human Papilloma Virus genotypes (16, 18, and 6/11) in Patients with Colorectal Cancer by DNA- Insitu Hybridization


Human papilloma viruses (HPV) have been detected in several types of cancers. Over the last few years, a possible correlation between HPV infection and colorectal cancer has been suggested. The aim of this study was to investigate the association between human papilloma virus (HPV) infection and colorectal cancer. To determine the relationship between HPV and colorectal adenocarcinoma, a retrospective study was done. This study was carried out on 50 patients with hisopathologically confirmed primary colorectal cancer. Two samples were collected from each patient: one sample from the tumor site and the other one from adjacent normally appearing colorectal tissues, as well as ten (20) colorectal tissues from control individuals with no cancer. In situ hybridization (ISH) was used to detect HPV DNA (HPV 16 and 18 DNA ISH and 6/11 DNA CISH) in colorectal tissues. HPV 16 was detected in 16(32%) of tumor samples, and in 7 (14%) of adjacent normal tissues, and in additionally two cases of apparently healthy group gave positive results for it. HPV 18 was detected in 11 (22%) of tumor samples and in 6 (12%) of adjacent normal tissue. HPV 6/11 was detected in 24 (48%) of tumor samples and in 7 (14%) of adjacent normal tissue.Our results suggest that colorectal HPV infection is common in patients with adenocarcinoma colorectal, albeit at a low DNA copy number, with HPV16 being the most prevalent type. HPV infection may play a role in colorectal carcinogenesis.