Effect of Environmental Contamination on Cell Division of Chick Embryos
Abstract
This study aimed to use chick embryos as a biological indicator for assessing the genotoxic activity of environmental pollutants and used it as a living test system or a useful tool when a study on the target species is difficult or impracticable. Fertilized eggs were collected from AL-Twietha(contains the Iraqi nuclear reactor)represents a control group . Cytogenetic analysis for chick embryos have been done by studying Blast index (BI), and Mitotic index (MI), Replicative index (RI) and Sister Chromated Exchange (SCE) for embryonic cell in 2-7 days age. Results were compared with those that were held to chick embryos for eggs collected from AL-Kadhimiya and AL-Baya as a control group. Result showed significant increase in MI and RI in embryonic cells for chicks collected from AL-Twietha with age were as embryonic cells for chicks collected from AL-Kadhimiya and AL-Baya that decreased as age increase. SCE values showed a significant increase for eggs collected from AL- Tuwaitha area. These results show a defect in the genetic material and inability of the cell to repair the DNA damage, which resulted in a rise in SCE value. From this study it can be conclude that the possibility of using chick embryos as a biological indicator to detect the effect of pollutants and adoption as an alternative method for in vitro blood cultures for being easy ,accurate, and economic method.
Keywords
Sister chromatid exchange, SCE, cell proliferation, chick embryos, environmental pollutantsMetrics