A new Approach for the Turbidimetric Determination of Cyproheptadine Hydrochloride in Pharmaceutical Preparations by Using Homemade Linear Array Ayah 6SX1-T-2D Solar cell CFI Analyser


A new turbidimetric – flow injection method is described for the determination of Cyproheptadine hydrochloride(CPH-HCl) in pure and pharmaceutical preparations .The method is characterized by simplicity ,sensitivity and speed ,it is based on formation of ion-pair between CPH-HCl and hexacyanoferrate (III) in aqueous medium to form yellow precipitate .This precipitate was determined using homemade Ayah 6SX1-T-2D Solar cell-CFI Analyser via the reflection of incident light from the surfaces precipitated particles at (0-180°) .Chemical and physical parameters were studied and optimized .The calibration graph was linear in the range of 0.5- 10 mmol.L-1 , with correlation coefficient r = 0.9785 ,while the percentage linearity (%r2) was %95.75 .The limit of detection (S/N=3) 280.72 ng /100 µL from the stepwise dilution for the minimum concentration in the calibration graph with RSD lower than %1.5 for 5and 7 mmol.L-1 (eight replicates ) concentration of CPH-HCl .The method was successfully applied for the determination of CPH-HCl in three different companies of pharmaceutical drugs . A comparison was made between the newly developed method analysis with classical method (HANNA instrument for turbidity measurement) using standard addition method via the use of t-test and F-test ,it was found that there was no significant difference between two methods at %95 confidence level .