Relationship of some hormonal and Ionic Constituents of ovarian follicular fluid with size of the follicle in local cows

Abstract

The purpose of this study was to examine the hormonal and ionic constituents of ovarian follicular fluid in relation to follicle size in local cows. The study was conducted at laboratories of department of animal technical production/ Al-Musaib technical collage, from September 2014 to February 2015. The ovaries were collected (120) ovaries from (60) non-pregnant cows which slaughtered at abattoir of province of Karbala. The ovaries were transported to the laboratory within 2hours post slaughter. Follicular fluid was aspirated from small (3-5mm), medium (6-10mm) and large (11-20mm) follicles, and stored at -5 Cº for further analysis. The follicular fluid samples were analyzed using commercial kits. The results show that the follicular progesterone and estradiol hormones concentration were significantly increased (P<0.05) with increase of follicular size. The difference in concentration of sodium between small and large follicles was non-significant, while was significantly higher (P<0.05) in large follicles. The difference in concentration of magnesium among three categories of follicles were non-significant. The results of the present of the study also indicated the concentration of calcium was significantly higher with increased of follicular size, while reverse the concentration of potassium was significantly lower (P<0.05) with enlargement of follicular size.