التنميط الوراثي لبعض العزلات الفطرية اعتمادا على التغاير في منطقة ITS ( internal transcribed spacer) كشفرة وراثية عالمية في تشخيص الفطريات

Abstract

This study aimed to use ITS ( internal transcribed spacer) region as a universal DNA barcode in identifying some fungal strains to confirm molecular diagnosis . Five strains from deuteromycetes class have been selected.Aspergillus niger .(2), Aspergillus flavus .(1) , Pencillum oxalicum.(1), Trighoderma harzianum.(1) , Ulocladium alternaria.(1) DNA of the five strains have been extracted, and the target region (ITS1 5.8S ITS2) of the rDNA has been amplified by using the universal primers ITS1 – ITS4. The gel photo visualized five bands in different molecular size between 496-625 bp. The difference in bands size refer to the genetic diversity among the strains which were as following, Trighoderma harzianum.(1) 625 bp, .Aspergillus niger .(2) 599 bp, Aspergillus flavus .(1) 595 bp, Pencillum oxalicum.(1)575 bp,. Ulocladium alternaria.(1) 496-498 bp. The study has been successfully identify the five strains according to the genetics differences in ITS region which Compatible with microscopic and morphological identification, thus, this study recommended using a molecular studies which is more accurate and quick identification compare with the traditional one in fungai