Detection of Tomato yellow leaf curl virus (TYLCV) and its strains on Tomato plants and host rang determine by Polymerase Chain Reaction

Abstract

This study was conducted to identify , determine the host rang, detection ofvirus strains and find out the source of initial infection of Tomato yellow leaf curlvirus (TYLCV) on tomato Solanum lycopersicom L. ,weeds and other economic cropsplanted closed to tomato fields in the desert region of Najaf province by polymerasechain reaction (PCR) technique. Samples of tomato leaves showed curling , rolling,stunting and yellowing symptoms were collected from the hybrids Aula,Aseel,Shreen and Abu- alkaf . PCR results showed that tomato plants have two strains ofTYLCV which were occupied area strain (Palestine ) TYLCV-IS and normal strainTYLCV-mld in Sheen hybrid, by amplifying two DNA bands with sizes of 634bpand 316bp respectively , where the other TYLCV -infected hybrids were given a634bp DNA band , in addition PCR results showed that the economic plants used inthis study Eggplant (Solanum melongen L.) , Melon ( Cucumis melo L.) , Cucumber( Cucumis sativus L.) , Sesame (Sesamum indicum L.) and Cowpea (Vigna sinensisL.) and Weeds plants Small flowered mallow (Malva parviflora L.) , Wall goosefoot(Chenopodium murale L.),and Kinoa (Chenopodium quinoa Willd.) , gave positiveresults with TYLCV-IS strain by amplifying a 634bp – DNA band representing theoccupied area strain. While Common sow thistle (Sonchus oleraceus L.) and Popsaltbush (Atriplex holocarpa F- muell.), gave negative result by PCR test.