Molecular Detection of Methicillin and Vancomycin Resistance in Staphylococcus aureus Isolated From Burn and Wound Infection Patients


Methicillin-resistant Staphylococcus aureus is the major cause of healthcare-associated bacteremia in most of Hospital and it increased risk of infection, morbidity and mortality especially, when associated vancomycin resistance in same infection. In this study 42 S. aureus were isolated from burn and wound infection patients in Al-Qadisiyah teaching hospital and S. aureus was isolated by selective medium out form 50 swab samples. The PCR assay was used for direct detection of methicillin (mecA) and vancomycin (van) antibiotics resistance gene in 42 S. aureus isolates. The results show 8 isolates (19%) were have mecA gene methicillin resistance and 3 isolates (7.1%) were have van gene vancomycin resistance in all isolates. In conclusion, PCR assay as highly sensitive and specific in detection of methicillin and vancomycin resistance gene, and Vancomycin-resistant can be associated with Methicillin resistance in S. aureus isolated from wound infection.