Real-time PCR Assay for Diagnosis of Eukaryotic Peptide Chain Release Factor Subunit 1 Gene in the Stages of Giardia duodenalis


Giardiasis caused by Giardia duodenalis is a common intestinal disease. Although this parasitic infection is found in mammals including human, pets and livestock. A total of 250 samples was collected from patients that were suspected the infection with Giardia duodenalis during the period from August 2014 to October 2015. The aim of this study is to investigate the molecular identification of Giardia duodenalis in patients suffered from diarrhea depending on a Eukaryotic peptide chain release factor subunit 1 gene to Confirm its existence in both stages of Giardia dudenalis (Trophozoit and cyst) and the possibility adopting it as a diagnostic gene. The results revealed that 200 samples were positive depending on the microscopic examination , 100 samples of stool have been chosen to isolate the cysts from it, while 100 samples of stool were used for trophozoits isolation , which in turn have undergone the third stage of the examination for the purpose of qualitative accurate diagnosis of Giardia using of quantitative Real Time PCR assay for amplifying the target region Eukaryotic peptide chain release factor subunit 1 and study the possibility of using the adiagnostic gene.