The inhibitory effect of celery, peppermint extracts and their mixture in comparison with amikacin on uropathogenic isolates: an in vitro study.


Urinary tract infections (UTIs) are regarded as amongst the most common serious health problems that affect millions of people each year. The antibacterial activity of two different plant extracts & their mixture at two different concentration (100 & 200 mg/ml) were carried out against two uropathogens Known as mainly bacterium induced urinary tract infections in humans & animals. The dried extracts of celery seeds and peppermint leaves and their mixture were tested in vitro against six isolates of E.coli & three isolates of K.peumoniae by agar well diffusion & micro-diluted method. Amikacin (10µg) was used as reference antimicrobial. The plants extracts and their mixture showed closed related, potent antibacterial activity and inhibition patterns varied mainly according to the concentration of extracts. Moreover, the E.coli & K.peumoniae showed nearly same sensitivity towards the studied plants in culture media and micro-diluted plate. The MIC was measured using standard two-fold microdilution broth methodology at concentrations ranging from 8 μg/ml to 4096 μg/ml. The MIC values of the ethanolic extracts of celery, peppermint & their mixtures were in the range of 256 to 512 µg/ml against E.coli and 128 to 512 µg/ml against K.pneumoniae.