Pathogenicity Study of a Number of Gram- Negative Bacilli Isolated from Clinical Samples

Abstract

Fifty isolates from different clinical samples (urine and wounds) were collected from Al-Nu'man General Hospital in Baghdad city. Sampling activates were carried out from 23April to 23June 2014. The age of patient range between 40- 70 years . The Gram-negative isolates were subjected to antibiotic susceptibility, β-lactamase production (86%), biofilm formation (84%), hemolysin production (48%).The resalt appered that Escherichia coli (54%) was the most common followed by Proteus vulgaris (18%), Enterobacter aerogenes (8%), Proteus mirabilis (6%), Enterobacter cloacae (4%), Acinetobacter baumannii (4%) and (2%) for each Klebsiella oxytoca, Enterobacter gergaviae and Pseudomonas mendocina. Gram negative isolates were observed resistance (100%) for Ampicillin, Carbenicillin, Cefalothine, Cefotaxim and Meropenem. Most isolated bicteria showed less resistance to Amoxicillin / clavulanic acid and Ceftazidime, also they showed sensitivity to Imipenem.Plasmid profile analysis, Proteus vulgaris isolates showed one large plasmid only.