Assessment of RBCs membrane protective activity of citicoline and eicosapentanoic- decosahexanoic acid in osmotic fragility model


Different organic compounds possess a good membranes stabilizing effects thatprotect cells like erythrocytes from strains exerted by change in medium osmolarity .These changes in tonicity could predispose RBCs for hemolysis . This disorder couldbe assessed by osmotic fragility test . From those compounds that widen osmoticfragility test values are citicoline (membrane phospholipids precursor) andpolyunsaturated fatty acids EPA-DHA.Assessment of these compounds on osmotic fragility test revealed that both ofciticoline and EPA-DHA will protect RBCs from hemolysis for a wider range thanvalues of the control blank. Although these effects were statistically not significanton considering statistical correlation test, however, the differences were important onconsidering t test for the range of saline concentration between 0.4-0.5 at P<0.05 .From the overall results, both citicoline and pufa induced a significant change instatbility of RBCs membrane upon exposure of osmotic effects in the salineconcentration between 0.4-0.5. Further evaluation of citicoline and EPA- DHA effectsmay be necessary before recommending the use these compounds in protectionagainst hemolytic diseases.