Molecular and bioinformitic analysis of ITS1 region of three Eimeria species in Kerbala and Babylon provinces, Iraq

Abstract

Abstract Global production of chickens has trebled in the past two decades and they are now the most important source of dietary animal protein worldwidE.Chickens are subject to many infectious diseases that reduce their performance and productivity. Coccidiosis, caused by apicomplexan protozoa of the genus Eimeria, is one of the most important poultry diseases. Understanding the biology of Eimeria parasites underpins development of new drugs and vaccines needed to improve global food security. A Fifteen of DNA samples (five samples for each one of three species) of Eimeria has been sequenced and analyzed in which multiple sequence alignment online based analysis for the ITS1 (Internal Transcribed Spacer 1) region that previously amplified by polymerase chain reaction, A comparison between the sequences of bases of local isolates of Eimeria with global isolates that recorded in Gens Bank and the comparative molecular screening of the present study results revealed the Validity and accuracy of diagnosis of three Eimerian species. Phylogenetic tree analysis using the program (MEGA 6) were adopted to determine genetic tree of the species analysis to compare the three of local species with global strains of Eimeria and found the Homology sequence identity of Eimeria tenella local isolates in comparison with NCBI-Gen bank Eimeria tenella (JX853830). Using NCBI-BLAST the results showed 98% and 99%, while the Homology sequence identity of Eimeria necatrix of local isolates in comparison with NCBIGenbank Eimeria necatrix (JX853832.1) were 91% and 100 % and the Homology sequence identity of Eimeria maxima of local isolates to NCBI-Genbank Eimeria maxima (JX853828.1) was 98%.