Spectrophotometric Determination of Paracetamol by Diazotization and Coupling Reaction with Anthranilic Acid


This paper concerned with development of a spectrophotometric method for the determination ofparacetamol, based on the diazotisation and coupling reaction with anthranilic acid in basic medium,to form an intense yellow coloured, water-soluble and stable azo-dye which shows a maximumabsorption at 421nm. Beer’s law is obeyed over the concentration range of 1.0-10 μg/ml; with molarabsorptivity of 2.1772×104 L.mol-1.cm-1 and Sandell’s sensitivity index 6.9446 μg.cm-2. Themethod has been applied successfully for the determination of paracetamol in pharmaceuticalformulation.