InVitro Multiplication of Shoot Tips and Nodes of Bauhinia alba L.

Abstract

Sterile seeds of Bauhinia alba L. cultured on MS media free from hormones after germination shoot tips and nodes were cultured on MS medium supplemented with Kin. at 0.0 , 2.0 , 4.0 , 6.0 , 8.0 mg/l or with BA 0.0 , 0.5 , 1.0 , 1.5 , 2.0 mg/l , data were taken after eight weeks , shoots produced in vitro cultured on MS full strength or half strength salts supplemented with IBA at 0.0 , 0.5 , 1.0 ,1.5 mg/l , data refers that multiplication of shoot tips and nodes were better when cultured on medium supplemented with BA compared with medium contained Kin. , highest shoots number 7.5 shoot/explant were obtained from cultured shoots tips of medium supplemented with1.0 mg/l BA , other wise highest shoots number 9.22 shoot/explant were achieved from cultured nodes on medium contained 1.5 mg/l BA after eight weeks , shoots produced in vitro gave highest percentage rooting 100% with average root number 10.5 root/explant and with length 12.17cm. from cultured on full strength salts MS medium supplemented with 1.0 mg/l IBA after four weeks , plantlets produced in vitro acclimatized in laboratory with survival 90%.