Effect of β-calcium sulphate hemihydrate on mandible healing in dog (radiographical assessment using Image-J Program)


This study was conducted to estimate the bony tissue response to β-calcium sulphate hemihydrate (CSH) as a bonesubstitute via radiographic assessment using ImageJ software. The extraction sockets in dog mandible were the regions ofinterest (ROI). Twenty adult (12-24 months), local breed dogs were included in the experiment. All had a complete set ofpermanent dentition. They were randomly allocated into four groups, each containing 5 animals. Bilateral lower thirdpremolars have been extracted. The right socket was filled with β-calcium sulphate hemihydrate, whereas no material wasplaced to fill that in the left side to serve as a control. Tissue response in extraction sockets was evaluated using two postoperativeintra-oral periapical radiographs for each tooth socket, the first immediately after extraction and the second at the endof each study interval (i.e., after 2 weeks, 4 weeks, 8 weeks, and 12 weeks period for group I, II, III, and IV, respectively). Theradiographs were converted from conventional to digital by X-ray scanner, then examined by ImageJ software. Radiographicassessment included the evaluation of differences in extraction sockets densities, bone resorption %, bone formation %, anddensity of the newly formed bone. The results showed significant differences between the left (control) and right(experimental) sides in all study periods in relation to differences in extraction sockets densities. Meantime, significantdifferences were noticed between right and left sides during a 12 week period in relation to bone resorption and boneformation %. Concerning density of the newly formed bone, significant differences were noticed during 8 week and 12 weekperiod.In conclusion, the use of β-calcium sulphate hemihydrate as a bone substitute significantly reduced bone resorption andincreased the rate of new bone formation. In addition, the density of the newly formed bone in the right (experimental) sidewas greater than that noticed in the left (control) side.