DNA Sequences Analysis of tox A Gene Isolated from Pseudomonas aeruginosa Bacteria.

Abstract

75 isolates of Pseudomonas aeruginosa was obtained from different clinical conditions. The result showed that 54 isolates which were related to P. aeruginosa have tox A gene will a ratio (72%), while 21 isolates will a ratio (28%) from the isolates didn’t have this gene and when we compared the duplicated bands with a suitable DNA marker, the size of the gene was found to be 352 base pair. The analysis of nucleotide sequence for tox A genes of P. aeruginosa was revealed there was some mutations in the DNA of this gene occurred in the nitrogen base sequence which almost included substitution type and insertion. The percentage of identities 96 and 99 % with the original gene. DNA sequencing analysis of amino acid translation of tox A genes revealed that most isolates display different point mutation as compared with NCBI data. Point mutation was detection in P. aeruginosa tox A causes conversion of Alanine to Threonine, Alanine to Glutamine, Alanine to Glycine, Leucine to Valine and Arginine to Leucine.