Detection the level of Heat Shock Protein70 (HSP70) and its relationship with puberty in female rats exposed to heat stress

Abstract

The study aimed to estimate the level of heat shock protein 70 (HSP70) and their relationship to puberty in female rats exposed to acute and chronic heat stress. Forty five (45) pups female rats divided randomly at weaning to 3 groups (15 females in each group). Group (1) (non-treated control group), group (2) (exposed to chronic heat stress) (38C°for 1 hour) daily from weaning to puberty, group (3) (exposed to acute heat stress (38C° for 4 hours at 35 days age for 5 successive days). Each of these groups are subdivided to 3 stages (pre, at, post puberty) (5 females for each stage). Serum samples from each stage (30days pre-puberty, and the age was differs at pubertal stage according to effect stress factor on time of puberty, 70 days post puberty) were used for detection of (HSP70) and estradiol (E2) by sandwich ELISA. Results showed no significant differences in (HSP70) level between the stages of all groups, while the estradiol significantly increase in group (3) in comparison with other two groups (2nd stage) and the estradiol showed significant increase in group (2), in group (1) and (3) (3rd stage). The first vaginal opening appeared in group (2) then group (3) and finally in group (1). The correlation between (HSP70) and estradiol was variant and swaying. Thus (HSP70) not affected by induced heat stress in female rats.