Ef ciency of nanoliposomes loaded with chloroformic extract of Jasminum sambac or Jasminin on the growth inhibition of some cell lines, a preliminary study

Abstract

The ef ciency of nanoliposome to deliver Jasminum sambac chloroformic extract or the puri ed compound Jasminin to cervical cell carcinoma )HeLa( cell line, rhabdomyosarcoma cell line )RD( and rat embryo broblast normal cell line )REF( was studded. Cells growth inhibition by the compound loaded liposome in compare to same unloaded compounds was determined. Nanoliposomes were prepared by the dehydration-rehydration method using Dimyristoylphosphatidyl- choline )DMPC(:Cholesterol as )50:45( mg/ml ratio and loaded with 50 μg/ ml of chloroformic extract of Jasminum sambac )which contain 1.11386 mg/ g of dried plant( or Jasminin separately. The encapsulation ef ciency of both chlo- roformic extract and Jasminin, liposome size, liposome surface charge and liposome shape was measured using HPLC, DSL and the scanning electron microscope )SEM( technique respectively. The results showed that nanoliposome was suc- cessful prepared by the suggested method and they were well loaded with the compounds. For nanoliposome which was loaded with the chloroformic extract the average size was 84.7 nm and zeta potential was -44.84. For nanoliposome which was loaded with Jasminin compound its average size was 70.3 nm and its surface charge was -57.00. The scanning elec- tron microscope )SEM( shows the spherical shape of liposome. The liposome encapsulation ef ciency for the chlroformic extract and jasminin were 40% and 50.84% respectively. Cancer cell line growth results indicated that both cloroformic extract and jasminin were capable of inhibit HeLa and RD cells up to 58% in concentration reange from 7.81 to 125 μg/ml after 72 hour of exposure time. However when using the nanoliposom encapsulated compound for both cloroformic ex- tract and jasminin the cancer cells growth inhibition elevated to highest growth inhibition percentage, for HeLa cells it was 84.31% and for RD cells it was 80.57% after 48 hours of exposure time. These records didn’t get higher signi cances after 72 hour of exposure time, inhibition percentages were 80.90% and 87.18% for HeLa and RD cells respectively. Results also indicated that there was no high inhibitory effect for DMPC, cholesterol and unloaded liposome on cell lines under investigation. In conclusion the encapsulation of the chloroformic extract and jasminin by the synthesized nanoliposome enhanced the capability of these compounds to inhibit cancer cells growth more ef ciently.