The Significance of TP53 Gene Polymorphisms as A Risk Factor For Non-Hodgkin’s Lymphoma in Iraqi Patients


Genetic factors including single nucleotide polymorphisms have been implicated as predisposing factors for large numbers of malignancies. When genetic disorders occur in a tumor suppressor gene, like TP53 gene, the results are expected to have devastating effects. The current study aimed to assess the effect of certain polymorphisms in TP53 genein the individual’s susceptibility to non- Hodgkin’s lymphomas among Iraqi patients. A total of 62 patients with these malignancies and 34 apparently healthy individuals were enrolled for this study. DNA was extracted from blood samples and fragment in TP53 corresponding for TP53 p.Arg72Pro, TP53 p.Pro47Ser and r.13494 G>A polymorphisms were amplified using specific primers. Genotyping was performed with restriction fragment length polymorphisms. The results revealed significant association of TP53 p.Arg72Pro polymorphism in both heterozygous and mutant homozygous genotypes with incidence of NHLs, while both TP53 p.Pro47Ser and r.13494 G>A polymorphisms had no such association. These results strongly indicate the importance of proline allele of TP53 p.Arg72Pro as a predisposing factor for NHLs.