Serotyping and molecular detection of some resistance genes of Escherichia coli isolated from urinary tract infection children in Nasiriyah City


Identification of  UropathogenicEscherichia coli (UPEC) from urinary tract infections and molecular detection of some serotypes and determine some of drug resistant genes. 500 urine samples were collected from Mohammed Al-Moussawi hospital and Bint-Alhuda hospital from urinary tract infection children in Nasiriyah City From September (2014) to June (2015).  The positive bacterial culture were 306(61.20%) isolate, distributed to gram negative with 192 isolate (75.62%) and gram positive with 114 isolates (25.37%). The highest percentage of gram negative bacteria represented by Escherichia coli with 75 isolate (39.06%). Of a total of E. coli 50 isolates, were identifiy by convential polymerase chain reaction (PCR) for the isolated DNA by using specific primers for (O6, O15, O16, O25 and 16SrRNA) genes results showed that 36 (72%) isolate represent 57 serotype and 14(36%) The serotype were distributed as 23(40.35%), 18(31.57%), 7(12.28%), 9(15.7%) and 50(100%) respectively. The antibiotic resistant genes of Escherichia coli (CITM,tet(A), aac3-IV, Sul1, dfrA1). The results of the present study showed appositive reaction with 25(50.08%), 23(46%), 19(38%), 17(34%), 5(10%) respectively. The present study results some of E.coliSerogroups carry genes responsible  for its resistance to most antibiotics.