Study of Antibacterial Activity of Ocimumbasilicum Against Staphylococcus aureus in Vitro


The investigation of inhibitory effect of the alcoholic extract of Ocimum basilicum on the growth of Staph. aureus that was isolated from the skin infected in vitro have been studied. Ocimum basilicum was isolated using 95% ethanol. Out of which the percentage of extraction of 45% of weight of dried powder was prepared in ascending gradient concentrations of the alcoholic extract (10-100 mg/ml) and the effective one was selected by agar diffusion method using Staphylococcus aureus. The diameters of the inhibition zones of the bacterial growth were increased parallel with the concentrations of the alcoholic extract concentrations. Low efficiency detected post using 10-20mg/ml concentrations, medium efficiency post using 40-60 mg / ml, whereas concentrations of 80- 100 mg/ ml were highly effective and influential against growth of the Staphylococcus aureus.