ISOLATION, IDENTIFICATION OF SOME FUGAL ISOLATES AND TESTING THEIR ABILITY FOR LIPOXYGENASE PRODUCTION

Abstract

Eighty three local isolates of fungi were isolated from different resources(Peanuts , maize, rice, wheat, bread , domestic cheese of sheep, Milk local Cream,Iranian cream, Roquefort cheese and soil). These isolates were purified andidentified, it include 14 isolates of Aspergillus flavus, 13 Aspergillus niger,8Aspergillus terreus,3Aspergillus parasaticus,3Alternariaspp.,15Penicilliumspp.,7Fusarium spp.,5 Trichoderma spp., 11 Rhizopus spp. and7Mucor spp. The ability of isolates for producing aflatoxin were tested, the toxicisolates(Aspergillus flavus,Aspergillus terreus ,and Aspergillus parasaticus) wereremoved.Aspergillus niger which was isolated from maize was choosing as the bestlipoxygenase producer after Primary and secondary screening. The growth of theselected isolate colonies had the largest proportion than the Colonies of Penicilliumsp. and Trichoderma sp..all so the same isolate had high enzymatic activity801.4units/ml, while Penicillium sp. and Trichoderma sp. had (559.2 and 120)units/ml respectively.