The Role of Helicobacter pylori CagA infection on the presence of Bcl-2 marker in gastric carcinoma by immune-histochemical analysis and in situ hybridization technique


Background: Bcl-2 plays a major role in the process of apoptosis and their dysfunction underlies carcinogenesis.Objectives: The study objective was to assess the expression of Bcl-2 in gastric cancer cells in correlation with clinic-pathological parameters and to determine the effect of H. Pylori infection on the expressions of Bcl2 in gastric adenocarcinoma.Methods: Using immune-histochemical analysis and in situ hybridization technique were used to measure Bcl-2 protein and CagA H. pylori gene expressions respectively and its relation to clinic-pathological parameters were observed.Results: The results revealed that a significant differentiation between Bcl-2 expression on the patients with gastric cancer and control group. Also we found thatBcl-2 expression significantly higher in intestinal type in comparison to that diffuse type (p<0.001). But no significant association between positive Bcl-2 expression and the presence of CagA-H. pylori in patients with gastric carcinoma (p>0.05).Conclusion: Bcl-2 expression in gastric cancer patients was suggested of its association with gastric carcinoma especially intestinal type. Thus, detection of Bcl-2 markers might be a useful early diagnostic in gastric cancer. H pylori enhance Bcl-2 protein levels which cause deregulation of these apoptosis-associated genes that may play a role in the development of gastric adenocarcinoma