Effect of Aqueous and Ethanolic Extract of Aerial Parts of Parsley (petroselinum crispum) on Some Bacteria Isolated from Nosocomial Infections in-vitro


Parsley (Petroselinum crispum) is important medicinal plant, has widespread consumption in food. The current study is aimed at declaration of antibacterial activity of aqueous and ethanolic extract of P. crispum aerial parts (stems and leaves) in vitro against five bacteria; Shigella flexneri, Acenitobacter baumani, Morganella morgani, Enterococcus faecalis and Providencia spp. isolated from different specimens of nosocomial infection. Aerial parts were extracted employing maceration method, antibacterial effect investigated by agar well diffusion method by following concentrations (0.05, 0.1, 0.5, 1, 5, 10, 25, 50, 100, 150, 200 mg/ml) of each extract, compared with 10 µg/ml of ciprofloxacin and tetracycline as positive controls that were evaluated by agar disc diffusion method. Inhibition zones surrounding all extract concentrations and positive controls were measured. The influence of ethanolic extract exhibited zones of inhibition through (5-25) mm, while the inhibition zones resulted from the action of aqueous extract were between (5-27) mm generally. The extracts displayed perfect potency toward investigated bacteria as a whole. The aqueous extract was better than ethanolic extract on the strength of activity overall. In most cases, the effects to be based on rising the extracts concentrations. The 50 mg/ml concentration extracts was responsible for initiation the action as far as 200 mg/ml concentration altogether. The influence of maximum concentration (200 mg/ml) of the extracts was superior or likes to that of ciprofloxacin upon particular some bacteria. According to existing investigations it can be figuring out that the parsley aerial parts have effective antibacterial activity supporting their uses in traditional medicine, permitting their employment for treatment of nosocomial infections.