A Study of Some Blood Picture Parameters during Experimental Infection with Eimeria tenella in Local breeds and Foreign of Chicken

Abstract

The study was conducted in Animal house of the department of Biology, College ofEducation, Al-Qadisiya University during the period from 1/9/2013 to 1/9/2014. The studydiagnosed the parasite of E.tenella in naturally infected chickens in poultry farms. E.tenella wasisolated from the infected cases in Vet. hospital and Vet. clinics.Then,the cases diagnosed initiallyin the laboratory direct examining stool by ordinary microscope and the positive cases wererecorded and diagnosed by using the technique of Conventional Polymerase ChainReaction(PCR). There are 315 cases of infected chicken which are examined and the resultsshowed that (186 out of 315) stool Samples,59%, The experimental infection for each experimentwas done in age 21 day with dosage of (5×104) grown oocysts/chicken for E.tenella in two typesof chickens(Ross and local breed) Also,two groups of uninfected chickens (control) are used forthe sake of comparison.Hematological parameters in Ross chickens showed significant decrease (p<0.05) in fourthweek of infection), percentage of Packed cell volume (PCV)(18.47% in the fifth week ofinfection) and Red blood cell (RBC) count was (1.58x106 in the fourth week of infection) . Also ,the infection led to significant difference of white blood cell (WBC) count (30.19x103 in theseventh week of infection) comparison of uninfected chickens of the control group. While Thehematological parameters in local breed showed significant decrease (p<0.05) in Hbconcentration and percentage of PCV (7.13mg/100ml , 21.26% respectively in the fifth week ofinfection) , while the RBC count was (2.05 x 106 in the fourth week of infection and significantincreased (p<0.05) in WBC count was (30.63 x 106 the sixth week of infection) in comparisonwith the chickens of the control group.