Synthesis, Determination and Antibacterial Activity Studies of Trace Amount of Cu(II) and Ni(II) with New Reagent 2-[2`-Iodophenyl azo)]-4,5-diphenyl imidazole by Spectrophotometric methods

Abstract

New reagent and two complexes of general formula [ML2CL2].H2O, where M = Cu(II), Ni(II),L = 2-[(2`-Iodophenyl azo)]-4,5-diphenyl imidazole have been prepared in ethanolic solution.Solid compounds were isolated and characterized by elemental analysis and vibrational spectra. The above newly synthesized compounds were investigated for their antibacterial, antifungal activities as well as, a sensitive and selective method has been developed for the determination of micro amounts of Ni(II), Cu(II). The method is based on the chelation of metal ions with 2-[(2`-Iodophenyl azo)]-4,5-diphenyl imidazole (2-IPAI) to form an intense color soluble products, that are stable and have a maximum absorptions at 566 nm and at 534 nm, and εmax of 1.26*104 and 1.66*104 L.mol-1.cm-1 for Ni(II), Cu(II), respectively. The stability constants and relative errors and a relative standard deviations for Ni(II) and Cu(II) were: 3.134*1010, 5.022*1010 L2 mol-2 and (-1, -1.33%), (-0.6, 0.3%) and (0.31, 0.699%), (0.29, 0.63%) respectively.