Isolate the bacteria Pseudomonas aeruginosa from different clinical samples and study the effect of pyocyanin pigments of some pathogenic bacterial


Three hundred clinical sample were collected from patients admitted and comers to(Azadi Teaching, Kirkuk General, kidsGeneral) hospitals, 140 sample from females withpercentage (46.6%) and 160 sample frommale (53.3%), and through it found that 24samples with percentage (17.14%) represented positive for bacterial growth in females and26 samples with(16.25%) represented positive bacterial growth of male cases. The ability ofPseudo. aeruginosa to produce pyocyanin on various media were show that 43 clinicalisolates of Pseudo.aeruginosa (86%) produced pyocyanin on two media (Nutrient agar,Muller hinton agar) and 15 (30%) produced the pigment on Macconky agar and 24 isolates(48%) on blood agar. While the effect of pyocyanin pigment on the growth of differentpathogenic bacterial isolates the results showed that a gram positive bacteria such asStaphylococcus aureus was more sensitive to the pyocyanin pigment than gram negative bacteria (E.coli, klebsiella pneumonia) when grown on Nutrient agar or Muller hintonagar.