Embryonic development following the insemination with epididymal sperms of vasectomized mice activated invitro using Glycyrrhiza glabra (Licorice) extract: model for obstructive azospermia

Abstract

BackgroundMany studies found that the Glycyrrhiza glabra (G. glabra) showed a positive effect on the activation of sperm in vitro specifically improve the activity of progressive movement. However, it was not known the impact of this stimulant on the certain sperms parameters in vasal obstructive mammals.ObjectivesThis experiment was designed to examine the possibility of fertilization ova by epididymal sperm of vasectomized mice activated by G. glabra and study the early embryonic development, as a model for obstructive azoospermiaMethodsGlycyrrhiza glabra aqueous extract (0.3 mg/ml culture medium) was used for in vitro direct sperm activation technique. The superovulated female mice (SUO) were divided into two groups, the first group; the insemination of flushed ova was performed with epididymal sperm of vasectomized male mice activated by adding30% of G. glabra to the culture medium (treated group). While the second group, the ova were inseminated by epididymal sperm of vasectomized male mice activated by G. glabra - free medium (control).ResultsThis study showed a highly significant (P <0.01) improvement in certain sperm function parameters i.e the sperm forward movement , morphologically normal sperms (MAS) . There was a significant (P<0.05) increase in fertilization rate (FR) of SUO mice oocyte using 30 % G. glabra medium(75.84%) compared to G. glabra­ Free medium (Hams -F12 medium ) alone (54.43%).By adding 30% G.glabra to the medium , the number of 3-cell and 4-cell embryonic stage of SUO mice was significantly (P <0.05) higher than that of grouping G. glabra- Free medium mice embryo( 62.10 % and 65.78% vs.58.88 and 66.29% , respectively ).ConclusionThis study showed a highly significant (P <0.01) improvement in certain sperm function parameters i.e the sperm forward movement , morphologically normal sperms (MAS) . There was a significant (P<0.05) increase in fertilization rate (FR) of SUO mice oocyte using 30 % G. glabra medium(75 .84%) compared to G. glabra­ Free medium (Hams -F12 medium ) alone (54.43%).By adding 30% G. glabra to the medium , the number of 3-cell and 4-cell embryonic stage of SUO mice was significantly (P <0.05) higher than that of grouping G. glabra- Free medium mice embryo( 62.10 % and 65.78% vs.58.88 and 66.29% , respectively).