Isolated and Identification of some pathogenic bacteria in children with diarrhea and respiratory tract infection

Abstract

The main objectives of the present study were to determine the isolation of main pathogenic bacteria that cause diarrhea and respiratory tract infection in children under 5 years of ageby using specific parameters. Bacteriological analysis showed that from 50 stool specimens ; 21(42%) were strains of Escherichia coli others are Klebsiella pneumonia 9(18%) , Enterobacter cloacae 8(16%) and other Enterobacteriacea , and the distribution of diarrheal children by age . It shows that diarrhea is statistically associated with age and majority of cases occurring in children between 7months and under 5 years of age.Bacteria were isolated from 15 of 47 (31.91%) nasal swabs Staphylococcus aureus was most common , isolated in 10 out of the 47 (21.3%) nasal swabs collected from the children followed by E. coli , 9 (19.14%) β-hemolytic Streptococcus , 6 (12.3%) Klebsiella pneumonia . Other bacteria isolated in fewer specimens were Enterococcus faecalis (10.63%) and Enterobacter spp.(4.25%) .In this study, bacteremia was confirmed in 28(56%) patient out of 50 children. the most common primary infection was identified in 50(33.3%) from 150 samples: gastroenteritis and pneumonia 47(31.3%) .The most common pathogens were Streptococcus spp. (35.7%) especially Beta hemolysis Streptococci mainly isolated from cases with pneumonia. Escherichia coli was isolated in (17.9%) of the cases ; it was reported to be the most frequent gram-negative bacterial species recovered from blood cultures , Klebsiella pneumonia presented (14.3%) of the cases , Enterococcus faecalis and Enterobacterspp. Were isolated from blood cultures of three children (7.14% , 3.6%) who had been hospitalized for > 10 days.