Amplification and sequencing of hla and sea genes in Staphylococcus aureus isolated from outpatients in Nassyriah City


An increasing concern about the role of Staphylococcus aureus as an opportunistic pathogen that can affect different parts of human body. The pathogenic role of this bacterium is associated with its ability to secrete a highly effective virulence factors. Among these, the production of hemolysin a (hla) and enterotoxin A (sea). The aim of the current study was to investigate the occurrence and molecular profile of hla and sea genes in S. aureus isolated from Otitis media outpatients in Nassyriah city, Iraq during the period from August 2014 to January 2015. A total of 100 S. aureus isolate were assayed for the presence of hla and sea genes by Polymerase chain reaction technique. The study results showed that 68/100 and 52/100 isolate were positive for the two targeted genes, respectively (P≤0.05). five of S. aureus isolates (two for hla assigned IQSH1 and IQSH2 in which they granted the official Genbank accession numbers of KY468500 and KY468501, and three for sea assigned ZWS1, ZWS2 and ZWS3) were subjected to partial DNA sequencing to reveal their relative to similar isolates in Genbank. The phylogenetic tree that was created in MEGA7 software showed that there were different molecular relationships among the local S. aureus isolates with similar ones around the world. These findings may be lead to better understanding about the importance of S. aureus epidemiology in Iraq which began to increase steadily.