Histological Study of Specific IgG intake in the treatment of broiler chicks Ross 308 experimentally infected with Eimeria maxima


Eimeria maxima was Isolated locally by taking samples from Ileum and Jejunum area of 1050 of slaughted chickens in Ramadi city. After purification and sporulation in vivo, diagnosis was carried out depending on the shape and size of the parasite and the infected site. Diagnosis was also confirmed in Disease Diagnosis Laboratory in veterinary college/ Uni. Baghdad. Challenge strain was obtained by strengthening and increasing the isolate (five times) in Ross 308 type. Parasite was breaking by ultrasound Sonicater then Sporocyst and Sporozoite were extract, Challenge strain was injected in Albino Rabbet in different areas of the body after mixing with Freund solution's incomplete oil adjuvant. IgG was extracted from rabbet serum daily and was dialyzed. The extracted protein was then migrate by Electrophoresis with standard proteins. IgG was separated using Column Chromatography packaged with Sephacryl gel S300 HR height 95 cm and a diameter of 1.6 cm. Molecular weight was estimate by separating Blue Dextran 2000 dye. Seven proteins of different molecular weights were used to work the standard curve. IgG sample was Spectra Scanned to determine the appropriate wavelength for detection the highest peak measured. The highest peak wavelength was obtained at 280 nm (0.749). Extracted IgG concentration was measured by using the Spectrophotometer a wavelength of 280 nm. A concentration of 5 mg/ ml of IgG was ran using Electrophoresis along with standard IgG to detect Gamma globulin. ELISA was conducted for IgG to determine its concentration. IgG samples were stored at -4 °C. Results showed histological changes among samples compared to normal tissues. In addition, the best treatment found were using injections of Amprolium, then by inoculating Amprolium by mouth with anti acid compared to negative and positive controls