concentrations of salt on rot seeds and content of wheat leaves at 28 days of total chlorophyll and proline

Abstract

Rhizoctonia solani was isolated using plant traps and Trichoderma harzianum by dilution method from fields and drainage cods in the counties of Al-Haffar, Al-Eqbariah, Al-Dassim and Al-Chabab belong to the districts of Abbasiya, Hurriayh, Hirra and Mashkhab in the province of Najaf, respectively, to find out the effect of filtrates five isolates of the fungus Rhizoctonia solani which are ( R.s1, R.s2, R.s3, R.s4 and R.s5) and four isolates of the fungus Trichoderma harzianum which are (T.h 6, T.h 7, T.h 8 and T.h 9) developing in the four circles of salt CaSo4, MgSo4, MgCl2 and NaCl and at different concentrations and at 28 days in the percentage of rot the seeds of wheat and the content of wheat leaf of total chlorophyll and proline. The study showed that isolates R.s 5 for concentration (34.295) and (38.295) gm.ltr -1MgCl2, isolates T.h 6 for concentration (0.783) gm.ltr -1 CaSo4, isolates T.h 6 T.h 7 of the concentrations (38.295) gm.ltr-1MgCl2, and concentrations of (34.295), (36.295) and (38.295) gm.ltr -1 MgCl2(without fungi) led to rot seed compared to control (without salt), which amounted to 51.66% and the comparison (with fungi Broth), which amounted to 56.10 , 48.17 , 49.66 and 38.71% of the isolates and concentrations respectively. Also the isolate T.h 7 in a concentration (0.0028) gm.ltr-1MgSo4 to increase the content of total chlorophyll in the leaves of wheat which reached to 2.56 mg.g-1 compared to control (without salt) and (with fungi Broth) 2.10 mg.g-1 for both of them, while the isolate of R.s 1 in concentration (0.983) Gm.ltr-1 CaSo4 gave the highest amount since Prolin was 131.25 Mm.g-1 compared to control (without salt), which amounted to 5.52 Mm.g-1 and (with fungi Broth), which amounted to 6.44 Mm.g-1.