EVALUATION OF ANTIGEN-BASED ENZYME IMMUNO-ASSAY IN REFERENCE TO DIRECT MICROSCOPY FOR THE DIAGNOSIS OF ENTAMOEBA HISTOLYTICA IN STOOL SAMPLES

Abstract

In the present study, we have been used TechLab Entamoeba histolytica II EnzymeLinked Immune Sorbent Assay (ELISA) test for detection of Entamoeba histolytica andcomparing it with microscopic examination.A total of 80 stool samples were collected from patients with intestinal amoebae,including 47 males and 33 females, the age between (1-60) years. The stool samples wereexamined by direct microscopy ,then we investigated E. histolytica specific adhesinantigen in stool samples using ELISA( TechLab E. histolytica II test , United States ofAmerica USA). Out of 80 stool samples examined microscopically 65 were infected withintestinal amoebae. The examination of the 65 (81.3%) positive samples by usingTechLab E. histolytica II ELISA test revealed that 13 (16.3%) were positive for E.histolytica while 2 (2.5 %) positive result using TechLab E. histolytica II ELISA kit wasdetected among the15 (18.8 %) microscopically negative samples. The study shows themajority of intestinal protozoa occur in males 36 (45.0%) and females 29 (36.3%), aswell in E. histolytica high percentage were record in males 9 (11.3%) and in females 6(7.5%), the results shows the highest percentage of intestinal protozoa as well E.histolytica at age group (30-39) (22.5%) (7.5%) respectively