Ulcerative colitis (UC) and celiac disease (CD) are autoimmune diseases. Perinuclearanti-neutrophil cytoplasmic antibodies (p-ANCA) have been described in patients withinflammatory bowel disease, mainly ulcerative colitis. However, their role in pathogenesisand diagnostic value are still controversial.The aim of this study was to assess the prevalence of certain perinuclear anti-neutrophilcytoplasmic antibodies (p.ANCA) including anti-lactoferrin, anti-lysozyme and anti-elastaseantibodies among patients with ulcerative colitis and celiac disease. This study was conductedduring the period from August 2012 to January 2013. Subjects enrolled in this study were 34ulcerative colitis patients (age range of 14-60 years with a mean of 31.29 years) and 34 celiacdisease patients (age range of 12-58 years with a mean of 28.59 years). All patients wereattending two Iraqi hospitals in Baghdad and Babylon cities who were subjected toendoscopic examination under the supervision of a gastroenterologist, for their primarydiagnosis. The primary diagnosis was supported by serological examination for IgA /IgGtissue transglutaminase (tTG) for patients with celiac disease. Another 34 healthy subjectswho were matching the patients groups in their age range and mean, were also enrolled in thisstudy as a negative control group. Male to female ratios were approximately similar in allstudy groups.