The protective effect of Trigonella foenum- graecum L. seeds extract in high fat diet-streptozotocin induced hyperglycemic mice


Diabetes mellitus is a chronic metabolic disorder which affects carbohydrate, protein, and fat metabolism. associated with high morbidity and mortality. characterized by hyperglycemia together with biochemical alterations of glucose and lipid metabolism. Hyperglycemia and hyperlipidemia result in development of oxidative stress, systemic and local inflammation which were an critical factors for degradation of β-cells, insulin resistance and type II diabetes .Herbal medicine is one of the therapeutic strategies in the management of diabetes mellitus. It is considered to be less toxic and have fewer side effects than synthetic ones .The objective of this study was to investigate the possible protective effect of Trigonella foenum- graecum L. seeds(TFG) extract in High Fat Diet ∕ Streptozotocin -induced toxicity in mice. The seeds of TFG were defatted by soxhelt apparatus with n-hexane ,then marc was macerated with ethanol to obtain total ethanol extract. Thirty two male albino mice were fed high fat diet for two week plus single dose of streptozotocin 120mg ∕kg intraperitoneally to generate typeII diabetes model with increased adiposity. The protective and hypoglycemic activity of seeds extract against toxicity and hyperglycemia induced was evaluated in mice. Glimepiride was used as a standard treatment .FBG, serum C-peptide ,TC , Triglycerides ,HDL, LDL levels were measured at the end of 28th day of experiment then the animal sacrificed and liver and pancreas sections were prepared and stained for histological evaluation. This study was resulted in that (TFG) seed extract cause; FBG, serum lipid profile in hyperglycemic mice significantly decrease, It also increase serum C-peptide ,Glutathione and decrease Malondialdehyde levels significantly compared to untreated group; this was associated with improving histological features that impaired during STZ exposure. In conclusion, Defatted seeds extract has potential anti-hyperglycemic and hepatoprotective effect with free radical scavenging ability against STZ-induced toxicity.