Competitive Inhibition Relationship Between Erythropoietin And Interferon-Gamma In Renal Failure Anemia

Abstract

This is an in vitro laboratory study. It was done in order to prove the competitive inhibition between interferon-gamma (IFN-gamma) and erythropoietin (EPO) in chronic renal failure (CRF) by fixing an inhibition elicited by EPO on IFN-gamma expression by mononuclear cells. These cells were already separated from fresh anticoaggulated blood specimens from nine CRF-patients and nine healthy persons.Then, were co incubated separately with mitogen, phytohemaggluttinin with and without EPO under standard incubation conditions. Thereafter, IFN-gamma level was measured by ELISA. Results have shown that EPO significantly inhibits IFN-gamma expression by mononuclear cells in healthy persons, p value < 0.05, while this inhibition is not significant in CRF-patients.