The detection of human papilloma virus- 16, in relation to P53 in oral squamous cell carcinoma by in situ hybridization

Abstract

ABSTRACT
Background: Squamous cell carcinoma of the oral cavity constituted a major health problem and can be a leading
cause of death. Several studies link high risk Human papillomavirus -16 to oral squamous cell carcinoma as an
important etiological factor. Several molecular markers have also been the subject of an intense research work,
trying to clarify their role in oral carcinogenesis. Of these, the cancer suppresser gene P53 has been extensively
studied. Aim of the study: 1. to detect the association of the molecular markers Human papillomavirus -16, in relation
to P53 in the oral Squamous cell carcinoma using in situ hybridization technique. 2. To correlate Human
papillomavirus -16 with clinical data (age, sex, risk factors grade &stage).
Material & Methods: Tumor tissues from 33 patients with newly diagnosed as oral squamous cell carcinoma & who
were surgically treated collected, formalin fixed & paraffin embedded. Sections on charged slides were made from
each tissue block and submitted to in situ hybridization technique utilizing Human papillomavirus -16, P53 cDNA
probes.
Results: Human papillomavirus -16 was localized by in situ hybridization specifically within the nuclei of cancer cell it
was detected in (27) (81.8%) of 33 oral squamous cell carcinoma patients. By using in situ hybridization P53 were
detected in (93.9%) cases. There was no significant correlation between the Human papillomavirus -16 &P53
positivity. Most of Human papillomavirus -16 positive patients were within the age group (50-59) years. However, there
was no significant correlation between Human papillomavirus -16 positive &age. No significant correlation was
found to sex &risk factors either. Most of cases were moderately differentiated squamous cell carcinoma (79%).
however no significant correlation was detected regarding histopathological grading or clinical staging with Human
papillomavirus -16, & P53.
Conclusions: Being highly associated with Human papillomavirus -16 must be searched for in cancer tissues of such
patients.
Keywords: Oral squamous cell carcinoma, Human papillomavirus -16, P53, in situ hybridization. (J Bagh Coll Dentistry
2009; 21(4): 80-85).