Study compound of Vitis vinifera seeds using High Performance Liquid Chromatography (HPLC) and study its inhibitory effect in some bacteria isolated from urinary tract infection.

Abstract

Qualitative and quantitative detection of the active compounds in the phenolic extract of Vitis vinifera seeds was conducted by using HPLC. Results showed it contains all of the following compound (Galic acid, Catechin, Epicatechin, Epigallocatechin Epicatechin gallate,Epigallocatechin gallate,Procyanidins).The result of chemical test of the active compounds showed that water and alcoholic extracts contained phenols,terpens,steroids and flavonides. Inhibitory effectiveness was evaluated for different concentration of phenolic,watery and alcoholic extracts of seeds include (2.5, 5,10,20,40 and 80) mg/ml separately against bacteria Klebsiella pneumonia and Pseudomonas aeruginosa Isolated from urinary tract infection by Agar well diffusion method،، The results showed high inhibition effect aganist all bacterial isolates with the effect of concentration and genus of bacteria type of extract.