Preparation and Characterization of TiO2 Nanoparticles and its Applications as Antibacterial Agents


TiO2NP’s were synthesis by sol-gel method at different calcination temperatures. The prepared TiO2NP’s were characterized by X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), and UV-Vis spectrophotometer. The antimicrobial activities of the prepared TiO2NP’s were investigative for two kinds of bacteria, (Gram-negative and Gram-positive bacterium. XRD results shows that the type of the TiO2 structure was anatase (A) at low temperatures, rutile (R) with rise of calcination temperature (Tc) into 800 ᵒC and mixed phases .SEM demonstrated that the size of nanoparticles seems larger and the accumulations appears clearly with raise calcinations temperature. The optical properties measured by the UV-Vis. Spectroscopy to compute the energy band gap for all phases around (3.75- 3.5 eV) for anatase and (3.4eV) for rutile. TiO2 nanoparticles in both phases showed excellent antibacterial activity against two representative bacteria, Pseudomonas aeruginosa and Staphylococcus aurous.