Effects of green laser therapy on healing of infected wound in mice.


Abstract:Background: Little studies focused on stimulation of healing process of infected wound in mice. The predominant pathogens that infected wound were gram positive such as Staphylococcus aureusand gram negative such as P. aeruruginosa and Acinetobactor Baumannii. A. baumannii cause wide range nosocomial infections. Objective: study the influence of green laser 532nm at constant power density (irradiance) at different exposure times (5, 15) on healing of wound infected by Acinetobacter Baumannii created on BALB/C mice.Methods: An elliptical full thickness skin wound was created aseptically on a back of 45 adults, females BALB/C mice. The wounds were infected by Acinetobacter Baumannii and the mice were randomly divided into two sets, first one was infected ( non-irradiated controls)15 animals (five in each subgroup)dividing according to the days of irradiation, second set was infected(irradiated groups)randomly divided in to two groups, first group irradiated for 5 mints and second group irradiated for 15 minutes. Both irradiated groups subdivided according to follow up period 3, 5, 10 days. Animal killed on day 3, 5, and 10 after treatment and control group killed also on 3,5,10 after contamination. Cold laser therapy (CLT) achieved by semiconductor diode laser 532nm, output power =40mw, continuous wave laser, power density =5.71 mw/cm2 and doses 1.7J/cm2, 5.14J/cm2. The output of green laser fitted with beam expander to irradiate a circular area of diameter 7cm2. CLT started after surgery and repeated for 3, 5, and 10 days. Wound healing studied by calculating the percentage of wound closure and histopathological evaluation. Conclusion: the present study showed that green laser therapy had obvious influence on healing of infected wound especially with a dose 5.14 J/cm2.