Isolation and Identification of some Species of Bacterial Pathogens from Musca Domestic and Test their Susceptibility Againts Antibiotics
Abstract
Abstract The current study was conducted during the period from the beginning of October 2016 to April 2017 to isolate and diagnose the bacteria on the outer surface of the domestic fly (Musca domestica) and to determine the sensitivity of bacterial isolates against antibiotics using different agricultural mediums. Insects were collected from three sites in the Tikrit city which are: vegetable market, butchers market and sheep sheds. 50 samples were collected from each site. Bacterial isolates obtained from the external surface of insects were subjected to morphological and biochemical tests and API 20 E system was used for diagnosis.153 isolations were obtained belonging to 9 bacterial species . Escherichia coli reached 36 isolations that prevailed the rest of the bacterial species with an isolation rate of 23.5%, followed by Salmonella typhi 32 isolates by 20.9% , and Proteus mirabilis 31 isolates by 20.3% . Staphylococcus aureus has 25 isolations by 16.3%. Klebsiella pneumonia has 11 isolations by 7.2% , and Shigella spp has 7 isolates by 4.6%, followed by Citrobacter freundii which has 6 isolates by 3.9% , Then Hafina spp. Has 3 isolations by 1.9% , Serratia marcescens has only two isolates by 1.3% .The results showed that the highest contamination rate was in the vegetable market which is 92%, then the sheep sheds is 88%, and finally Butchers market is 86%. The Gram-negative bacterial isolates were tested for sensitivity to 8 types of antibiotics, E.coli showed the highest resistance to both Erythromycin and Ceftriaxon by 77.8% , while S.typhi showed resistance to Amikacin and Ceftriaxon by 53.1%, P.mirabilis was resistant to Tetracyclin by 64.5%, Shigella spp. was resistant to Ceftriaxon by 57.14%, K.Pneumonia bacteria were resistant to Erythromycin by 72.7% , C.freundii showed resistance to the antibiotic Trimethoprim + Sulfamethoxazol and 100%, Hafnia was 33.33% resistant to Chloramphenicol, Finally, Ser.marcescens were 100% resistant to Erythromycin.The sensitivity of Staph.aureus bacteria was tested against 5 types of antibiotics with the highest resistance to Vancomycin by 80% .
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