Detection of Biofilm Genes (IcaA and IcaD) in Staphylococcus spp.

Abstract

One hundred thirty one clinical bacterial samples were collected from skin, pus, urine and blood from patients at Al-Salam General Teaching Hospital in Mosul city during March until July/ 2012, The present study included identification using API ID 32 STAPH system to the species Staphylococcus. aureus, Staph. lugdunensis, Staph. epidermidis, Staph. hominis, Staph. auricularis and Staph. haemolyticus, Biofilm formation genes at icaA (188 bp.) were found in 35.7% of Staph. aureus, Staph. hominis, and Staph. xylosus but 42.8 % of Staph. aureus, Staph. epidermidis, Staph. hominis, and Staph. xylosus contained icaD (198 bp.) gene only.