Molecular and Serological Diagnosis of Buffalo's Brucellosis in Salahaldeen Governorate

Abstract

The aims of the study were to determined incidence ratio of buffalos brucellosis in Salahaldeen governorate and evaluating the efficiency of serological tests used in current study. For this purpose 135 buffalos (adult males, pregnant females and aborted females) were tested.From each animal two blood samples were collected, one for PCR test and other for serological tests (Rose Bengal test, tube agglutination test, mercaptoethanol test and ELISA test).The incidence ratio of buffalo brucellosis in Salahaldeen governorate were 14.8% in PCR test, while in serological tests were 21.4%, 13.3%, 16.2% and 7.4% for rose Bengal test, tube agglutination test, mercaptoethanol test and ELISA test respectively. Highest positive and negative agreement recorded between rose Bengal test and mercaptoethanol test were 34.9% & 63.7% . The current study recorded highest sensitivity recorded in rose Bengal test (75%), highest Specificity in ELISA (97.1%). Highest postive predictive values s and negative predictive values s recorded by tube agglutination test were: 77.7% and 96.2%