Determination of the Inhibitory Efficacy of Iraqi Tribulus Terrestris l. Fruit Extract Against Some Types of Staphylococcus Aureus and Detection of Its Active Compounds

Abstract

A total number of 50 Staphylococcus aureus (56.81)% from 88 isolates of Staphylococci were isolated and identified from wounds, burns, ear, urine, eyes, pus and skin boils, in laboratories of Maternity and Child Teaching Hospital, Ramadi Teaching Hospital, and Al-Yarmouk Teaching Hospital in Baghdad for the period from March to May/2017. Isolates diagnosis depended on microbial, cultural, and biochemical characteristics. Concentrations series of Tribulus terrestris ethanolic extract were prepared at concentrations of 3.125, 6.25, 12.5, 25, 50, and 100 mg/ml with inhibition zones of 0, 0, 11.3, 16.6, 21.3, and 25.6 mm respectively, minimum inhibitory concentration determined as 12.5 mg/ml. The results of GC-MS showed that extract contains many active chemical compounds most important which phytol, pyrrole, lupeol, taraxerone, saturated and unsaturated fatty acids that play essential role in growth inhibition of bacteria and have ability to reduce biofilm production, also many active groups were identified by using fourier-transform infrared spectroscopy (FTIR) technique. Results showed that ethanolic extract reduced the ability of S.aureus to produce the biofilm by using sub – MIC of 50 – 81.4 %.