Advanced Stimulatory Method for Activation of Sperm Function Parameters by using TAD Glutathione in Iraqi Infertile Men

Abstract

Background: One of the major concerns among the male reproductive health isthe inability to achieve parenthood even after regular unprotected intercourse.The efficiency of the sperm selection techniques is expressed as the concentrationof spermatozoa with normal motility. Discontinuous density gradientcentrifugation (DGC) method widely used over the last decade due to effectivenessand reproducibility in recovering most motile sperm, which separatessperm based on their motility, size, and density differential. Glutathione act asan amino acid donor during spermatogenesis. If the levels of glutathione are toolow during spermatogenesis, the number of mature and morphologically normalspermatozoa generated will be decreased.Objectives:The aim of this study was to assess the beneficial role of TAD 600mgGlutathione combined with DGC technique for activation of human sperm ofasthenozoospermic, oligozoospermic and normozoospermic subjects comparedwith the DGC technique alone.Patients, Materials and Methods:Sixty males were involved in this study, dividedinto three groups, (twenty asthenozoospermic, Twenty oligozoospermicand twenty normozoospermic subjects)during their attendance to the InfertilityClinic at High Institute for Infertility Diagnosis and Assisted Reproductive Technologies;Al- Nahrain University. Semen samples were obtained, and seminalfluid analysis was assessed. Each semen sample was divided into three parts.The first part prepared as in vitro sperm characterization before activation, thesecond part using density gradient centrifugation technique, while the last partprepared using density gradient centrifugation combined with TAD 600mg Glutathione.Results: After in vitro sperm activation for asthenozoospermic, oligozoospermicand normozoospermic samples a significant increase was observed in the spermfunction parameters including sperm concentration, motility and morphologicallynormal sperm percentage as compared to pre-activation using combined techniques(density gradient centrifugation and TAD 600mg Glutathione) as comparedto DGC technique alone.Conclusions:TAD 600mg Glutathione combined to the DGC technique was foundto give higher significant results on sperm function parameters (sperm concentration,motility and morphology) when using a low quality of semen samplessuch as decreased sperm motility as compared with DGC technique alone.